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Optimizing nanocarriers for targeted drug delivery: One of the main problems in cancer therapeutics is to be able to target cancer cells specifically with minima damage to normal tissue. We examine how a particular class of nano-carriers with multiple ligand bearing arms can be designed to optimize this selectivity.

Igor Goncharenko and Ajay Gopinathan (in preparation)

 

Novel forms of Chemotaxis:  Chemotaxis usually involves cellular motion towards an exogenous, often static, source of chemoattractant. We demonstrate an enhanced mechanism in which a single particle (the cell) releases a chemical that diffuses to fixed particles (targets) and signals the production of another chemical by the targets. This secondary chemical acts as the cell’s chemoattractant. To reach a single target, we describe optimal strategies that arise for the release of a fixed amount of probe chemical. In the presence of multiple targets, the one selected by the cell depends on the strength and, more interestingly, the frequency of probe chemical release. Although it involves one more step than standard chemotaxis, our simple chemical “pinging” mechanism can offer far greater flexibility in regulating target selection seen in a number of physical or biological realizations.

Sarah A. Nowak, Buddhapriya Chakrabarti, Tom Chou, and Ajay Gopinathan, "Frequency-dependent Chemolocation and Chemotactic Target Selection", Submitted to: PLoS Com. Bio., (2009)

 

In-vitro Filopodia: Filopodia are bundles of actin filaments that extend out ahead of the leading edge of a crawling cell to probe its upcoming environment. In vitro experiments [D. Vignjevic et al., J. Cell Biol.  160, 951 (2003)] have determined the minimal ingredients required for the formation of filopodia from the dendritic-like morphology of the leading edge. We model these experiments using kinetic aggregation equations for the density of growing bundle tips. In mean field in the absence of bending effects, we determine the bundle size distribution to be broad for bundle sizes smaller than a characteristic bundle size after which the distribution decays exponentially. Two-dimensional simulations incoporating both bundling and cross-linking measure a bundle size distribution that agrees qualitatively with mean field. The presence of bending effects yields a bundle size distribution that is peaked at a particular bundle size. Finally, two-dimensional simulations demonstrate a nonmonotonicity in the radial extent of the dendritic region as a function of capping protein concentration due to the interplay between percolation and the ratcheting of growing filaments off a spherical obstacle.

K.-C. Lee, Ajay Gopinathan, J. M. Schwarz, "Modeling the formation of in vitro filopodia", arXiv:0909.2594v1 (2009), Submitted to Journal of Theoretical Biology

 

Optimizing Yield Rates : The problem of optimizing the yield rate in reactions that are catalyzed by enzymes is of fundamental importance. In the case of protein folding catalyzed by chaperonins, there is a distinct possibility of the formation of misfolded proteins within the chaperonin nano-cage which can be considered as an undesirable intermediate state of the reaction. Chaperonins are known to nonspecifically release their contents in an ATP consuming reaction which suggests a mechanism for releasing undesirable intermediates. However, this also results in the release of proteins that are on the correct folding pathway. Balancing these two conflicting outcomes suggests the existence of an optimal release rate. This paper shows the existence of maxima in the yield rate at specific optimal values of the release rate of the undesirable intermediate state. We discuss the conditions for the existence of such maxima and their dependence on the other parameters of the system.

Igor Goncharenko and Ajay Gopinathan, “Optimal Kinetics for Chaperonin Assisted Protein Folding”, IAENG Transactions on Engineering Technologies 2, p13 (2009)
 

Igor Goncharenko and Ajay Gopinathan, “Optimal Yield Rates in Enzymatic Reactions with Undesirable Intermediate States”, Proceedings of the International Conference on Computational Biology (WCECS 2008), p24 (2008)

 

Diffusion and binding of finite-size particles in confined geometries : Describing the diffusion of particles through crowded, confined environments with which they can interact is of considerable biological and technological interest. Under conditions where the confinement dimensions become comparable to the particle dimensions, steric interactions between particles, as well as particle-wall interactions, will play a crucial role in determining transport properties. To elucidate the effects of these interactions on particle transport, we consider the diffusion and binding of finite-size particles within a channel whose diameter is comparable to the size of the particles. Using a simple lattice model of this process, we calculate the steady-state current and density profiles of both bound and free particles in the channel. We show that the system can exhibit qualitatively different behavior depending on the ratio of the channel width to the particle size. We also perform simulations of this system, and find excellent agreement with our analytic results.

Mark L. Henle, Brian DiDonna, Christian D. Santangelo, and Ajay Gopinathan, “Diffusion and binding of finite-size particles in confined geometries”, Phys. Rev. E 78, 031118 (2008)

 

Polymer Translocation in Crowded Environments : Polymer translocation is an extensively studied topic and is biologically an important process that occurs in a variety of circumstances where biopolymers (like DNA say from a virus) are transported across a membrane into a different environment (say the cell interior). An important question that arises is : How does the crowded nature of the cellular cytoplasm affect this process? While this has been addressed in the context of protein folding and biochemical rates in vivo, one would expect this to have a dramatic effect on translocation. We systematically treat the entropic penalty due to the crowded environment and find new power law scalings of the translocation time with polymer length. We also find that the crowding inflicts a significant barrier and that adding a chemical potential gradient in order to overcome this results in very interesting translocation regimes as a function of crowding, chemical potential and polymer length.

Ajay Gopinathan and Y.W. Kim, “Polymer Translocation in Crowded Environments” Phys. Rev. Lett., 99, 228106 (2007) 

 

Actin based propulsion :  A key process in motility is the production of a protrusive force that drives the cell’s leading edge forward. It has been well established that the cytoskeletal protein actin forms a dense, branched and crosslinked network of filaments at the leading edge and it is the polymerization of these filaments that gives rise to the force. The discovery that intracellular pathogens like listeria use the cell’s actin machinery to propel themselves played a vital role in establishing the biochemical basis of motility.  However, there still remains a hotly debated question: How does the polymerization activity at the molecular scale translate into a macroscopic force?  This led to the development of both the microscopic view that considers the protrusive force as arising from the monomer-by-monomer growth of a population of these filaments  and macroscopic models that treat the filaments as a continuum gel. Both of these describe the phenomenon albeit at very different length and time scales and are unable to satisfactorily account for all aspects of the motility (see for example the work by Kuo Lab. To get a correct and unified picture, we introduced a “dynamic gel” picture where we treated the actin network as an elastic gel modeled by a finite element mesh while allowing for spatial variations in polymerization activity .We treat the actin comet tail as an elastic continuum tethered to the rear of the bacterium. The interplay of polymerization and tethering gives rise to inhomogeneous stresses calculated with a finite element analysis. We quantitatively reproduce many distinctive features of actin propulsion that have been observed experimentally, including stepped motion, hopping, tail shape and the propulsion of flat surfaces.
 
Ajay Gopinathan and Andrea Liu, "Elastic Actin Tails: Shape, Stresses and Propulsion" in preparation

 

Cell Membrane Dynamics :
During cell motility, the leading edge of the cell exhibits a range of dynamic structures such as  lamellipodia (flat disk-like extensions), filopodia (thin needle like protrusions) and membrane ruffles (wave-like instability). These structures are like the “feet” of the cell and are put out in an effort to feel and make attachments to the surroundings. They are hence vital to the process of motility. These dynamic surface patterns of moving cells are usually observed to have length-scales in the micron range, and appear in many different cell types.  They occur usually when the cell is stimulated by an external signal, indicating that the receptors on the cell membrane have a role to play. The receptors actually turn on in response to the signal and in turn initiate actin polymerization activity in their vicinity, which leads to force production. Our recent work showed that the dynamic membrane structures described above can arise from a simple analytic model which couples the membrane dynamics to the dynamics of receptors that reside on the membrane and the actin based protrusive forces they generate. Our model predicted membrane ruffling and the onset of filopodia and also gave explanations for several experimentally observed puzzling features such as the increased microviscosity at the leading edges of motile cells and the temperature dependence of the ruffle velocities.

N. Gov, A. Gopinathan, "Dynamics of Membranes Driven by Actin Polymerization", Biophys .J, 90(2), 454(2006)

 

Cytoskeletal Kinetics : Controlling the polymerization activity of cytoskeletal actin network plays an important role in cell motility. Even in the absence of motility, the actin network is not static but evolves via kinetic processes such as actin polymerization, depolymerization, capping, branching and severing which are regulated by various proteins in the cell .
Abnormal levels of expression of these regulatory proteins lead to diseased states characterized by drastic morphological changes in the cytoskeleton and loss of function. It is therefore imperative to understand how the regulatory protein concentrations act in concert to maintain a normal cytoskeletal morphology. Previous work treated one or more but not all of the above mentioned processes. We recently studied the steady-state morphology of such networks and derived simple expressions for characteristics such as the length distribution of filaments and branches, branch spacing, and monomer to filamentous actin ratio as functions of regulatory protein concentrations. We found that these characteristics exhibit several scaling regimes with respect to the different protein concentrations and that the severing and branching activities are optimally coupled in the cell.
 
Ajay Gopinathan, J. Schwarz, K.C. Lee and A.J. Liu, “Branching, Capping, and Severing in Dynamic Actin Structures”,  Phys. Rev. Lett. 99, 058103 (2007)